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Index > Protein center > FGF2(Gene name) > Bovine
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  • FGF2 (Gene name),
  • Heparin-binding growth factor 2 (Protein name ),  FGF2_BOVIN from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • Gene name:
    FGF2(FGF-2);
    Protein name:
    Heparin-binding growth factor 2(HBGF-2);
    Alternative:
    Heparin-binding growth factor 2(HBGF-2);Basic fibroblast growth factor(bFGF);
    Organism:
    Bovine (Bos taurus). 
    General Annotation
    Sub Unit:
    Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2.
    Function:
    Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.
    Subcellular Location:
    Secreted Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane.
    Protein Attributes:
    Sequence length:
    155
    Sequence:
    50:
    MAAGSITTLP | ALPEDGGSGA | FPPGHFKDPK | RLYCKNGGFF | LRIHPDGRVD | 
    100:
    GVREKSDPHI | KLQLQAEERG | VVSIKGVCAN | RYLAMKEDGR | LLASKCVTDE | 
    150:
    CFFFERLESN | NYNTYRSRKY | SSWYVALKRT | GQYKLGPKTG | PGQKAILFLP | 
    155:
    MSAKS
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    SMR:
    String:
    Pfam:
    UniGene:
    Uniprot:
     
    FOR
    ELISA Kit for Bovine HBGF-2
    Cat.:
    E0551b
    Price:
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    MSDS:
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    Packing:
    96T
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    ELISA Kit for Bovine HBGF-2
    Cat.:
    E0551h
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    MSDS:
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    Packing:
    96T
    Range:
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    ELISA Kit for Bovine HBGF-2
    Cat.:
    E0551r
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    MSDS:
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    Packing:
    96T
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    ELISA Kit for Bovine HBGF-2
    Cat.:
    E0551m
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    MSDS:
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    Packing:
    96T
    Range:
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    ELISA Kit for Bovine HBGF-2
    Cat.:
    E0551c
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    MSDS:
    Please sign in first.
    Packing:
    96T
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    ELISA Kit for Bovine HBGF-2
    MSDS:
    Please sign in first.
    CLIA Kit for Bovine HBGF-2
    MSDS:
    Please sign in first.
    CLIA Kit for Bovine HBGF-2
    Cat.:
    U0551c
    Price:
    Please sign in first.
    MSDS:
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    Packing:
    96T
    CLIA Kit for Bovine HBGF-2
    Cat.:
    U0551r
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
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    CLIA Kit for Bovine HBGF-2
    Cat.:
    U0551b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Bovine HBGF-2
    Cat.:
    U0551h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Bovine HBGF-2
    Cat.:
    U0551m
    Price:
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    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Bovine HBGF-2
    Cat.:
    P0551Rb-h
    Price:
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Bovine HBGF-2
    Cat.:
    P0551Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Bovine HBGF-2
    Polyclonal Antibody for Bovine HBGF-2
    Cat.:
    P0551Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Bovine HBGF-2
    Polyclonal Antibody for Bovine HBGF-2
    Monoclonal Antibody for Bovine HBGF-2
    Monoclonal Antibody for Bovine HBGF-2
    Monoclonal Antibody for Bovine HBGF-2
    Monoclonal Antibody for Bovine HBGF-2
    Monoclonal Antibody for Bovine HBGF-2
    Monoclonal Antibody for Bovine HBGF-2
    Protein for Bovine HBGF-2
    Protein for Bovine HBGF-2
    Protein for Bovine HBGF-2
    Protein for Bovine HBGF-2
    Protein for Bovine HBGF-2
    Protein for Bovine HBGF-2

    R&D Technical Data
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    s

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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤4.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤9.8%

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    Recovery
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    Recovery was determined by spiking various levels of FGF2 into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    97

    92-100

    Plasma

    98

    96-107

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FGF2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    93-102%

    94-101%

    92-104%

    90-103%

    EDTA plasma(n=5)

    94-103%

    92-102%

    94-102%

    91-107%

    heparin plasma(n=5)

    92-104%

     

    90-107%

    93-104%

    95-102%

     

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    References
    1. 1.
      "Nucleotide sequence of a bovine clone encoding the angiogenic protein, basic fibroblast growth factor."
      Abraham J.A. , Mergia A. , Whang J.L. , Tumolo A. , Friedman J. , Hjerrild K.A. , Gospodarowicz D. , Fiddes J.C.
      Science233:545-548(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    2. 2.
      "Human basic fibroblast growth factor: nucleotide sequence, genomic organization, and expression in mammalian cells."
      Abraham J.A. , Whang J.L. , Tumolo A. , Mergia A. , Fiddes J.C.
      Cold Spring Harb. Symp. Quant. Biol.51:657-668(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    3. 3.
      "Primary structure of bovine pituitary basic fibroblast growth factor (FGF) and comparison with the amino-terminal sequence of bovine brain acidic FGF."
      Esch F. , Baird A. , Ling N. , Ueno N. , Hill F. , Denoroy L. , Klepper R. , Gospodarowicz D. , Boehlen P. , Guillemin R.
      Proc. Natl. Acad. Sci. U.S.A.82:6507-6511(1985) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 10-155
    4. 4.
      "Isolation of an amino terminal extended form of basic fibroblast growth factor."
      Ueno N. , Baird A. , Esch F. , Ling N. , Guillemin R.
      Biochem. Biophys. Res. Commun.138:580-588(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 10-19
    5. 5.
      "Isolation of fibroblast growth factor from bovine adrenal gland: physicochemical and biological characterization."
      Gospodarowicz D. , Baird A. , Cheng J. , Lui G.M. , Esch F. , Bohlen P.
      Endocrinology118:82-90(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 10-23 AND 25-38
      tissue: Adrenal gland.
    6. 6.
      "Isolation and partial molecular characterization of pituitary fibroblast growth factor."
      Bohlen P. , Baird A. , Esch F. , Ling N. , Gospodarowicz D.
      Proc. Natl. Acad. Sci. U.S.A.81:5364-5368(1984) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 12-24
      tissue: Pituitary.
    7. 7.
      "Isolation, characterisation and tissue localisation of an N-terminal-truncated variant of fibroblast growth factor."
      Bertolini J. , Hearn M.T.
      Mol. Cell. Endocrinol.51:187-199(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 14-33
    8. 8.
      "Isolation and partial characterization of an endothelial cell growth factor from the bovine kidney: homology with basic fibroblast growth factor."
      Baird A. , Esch F. , Boehlen P. , Ling N. , Gospodarowicz D.
      Regul. Pept.12:201-213(1985) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 25-41
      tissue: Kidney.
    9. 9.
      "Purification and partial characterization of a mitogenic factor from bovine liver: structural homology with basic fibroblast growth factor."
      Ueno N. , Baird A. , Esch F. , Shimasaki S. , Ling N. , Guillemin R.
      Regul. Pept.16:135-145(1986) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 21-40
      tissue: Kidney.
    10. 10.
      "Isolation and partial characterization of basic fibroblast growth factor from bovine testis."
      Ueno N. , Baird A. , Esch F. , Ling N. , Guillemin R.
      Mol. Cell. Endocrinol.49:189-194(1987) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 25-39
      tissue: Testis.
    11. 11.
      "Structural characterization of the fibroblast growth factor-binding protein purified from bovine prepartum mammary gland secretion."
      Lametsch R. , Rasmussen J.T. , Johnsen L.B. , Purup S. , Sejrsen K. , Petersen T.E. , Heegaard C.W.
      J. Biol. Chem.275:19469-19474(2000) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH FGFBP1
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